| Congresso Brasileiro de Microbiologia 2023 | Resumo: 730-1 | ||||
Resumo:The increase in the use of antibiotics in clinical and veterinary practice and the consequent increase in resistant bacteria generate a greater dissemination of both resistant bacteria and antibiotics in wastewater. The discharge of untreated effluents into bodies of water thus leads to a particular type of pollution that is little considered in our region. Hospitals represent critical points for the selection of clinically relevant resistant bacteria, given the amount of antibiotics and antiseptics used and released into the environment. The objective of this work is to evaluate the presence of coliforms resistant to beta-lactam antibiotics in hospital effluents from Buenos Aires, Argentina. To carry out this work, samples of wastewater from a pediatric hospital and acute care hospital were used, at different times of the year. Bacterial resistance to antibiotics was determined through the agar dilution method. To calculate the percentage of resistant bacteria (coliforms and Escherichia coli), dilutions of the samples were inoculated in the CHROMagar ECC medium with and without antibiotics. The following were used: ceftriaxone, ceftazidime, imipenen, meropenen, colistin. Antibiotic susceptibility testing and extended-spectrum beta-lactamase (ESBL) phenotypic detection have been performed following CLSI (2023) guidelines. Coliform bacteria resistant to all the antibiotics tested were detected in the effluents samples from the pediatric hospital. A high prevalence of coliforms resistant to ceftazidime (84.43%) followed by ceftriaxone (20.55%) was observed. On the other hand, in the acute care hospital the highest prevalence is observed with respect to colistin, detecting 6.20% of resistant coliforms and 25.00% of resistant Escherichia coli. Among the E. coli strains isolated, multiresistant strains producing extended-spectrum beta-lactamases (ESBL) were detected at the pediatric hospital. Klebsiella ssp pneumoniae strains with the ESBL phenotype were also isolated. In the acute care hospital, beta-lactamase-producing strains of Serratia liquefaciens and Enterobacter cloacae were isolated. Our results highlight the need to establish effective policies to reduce this growing public health problem.
Palavras-chave: beta-lactamases, coliforms resistant, hospital wastewater, public health Agência de fomento:Secyt UBA | |||||